ISIS Neutron and Muon Source Data Journal

Tuneable-Membrane Protein Containing Floating Bilayers: Enhancement of Complexity Resolution through Differential Hydrogen Labelling

Abstract: We have developed self-assembled floating supported membranes which contain intergral membrane proteins such as the COVID receptor angiotensin converter enzyme 2 (ACE2). This biologically accurate and easy to assemble sample system is self-assembled onto a Oligo-Ethylene-Oxide (OEG) Self-Assembled Monolayer (SAM) coated gold surfaces using a two step self assembly process. The distance of the resulting floating protein-lipid membranes from the bulk interface can be tuned by changing the solution salt conditions. we are at a stage in this work where we are preparing for publication but require some final data sets where the compositional complexity of the floating membranes is abundantly clear. There for here we wish to undertake some final measurements using lipid and protein deutertation to enhance the clarity of the protein distributions across the membranes.

Experimenter: Dr David Hardy
Experimenter: Dr Tim Knowles
Local Contact: Dr Luke Clifton
Experimenter: Professor Mark Sansom
Experimenter: Mrs Laura John
Experimenter: Dr Stephen Hall
Experimenter: Mr Nicolas Shiaelis

DOI: 10.5286/ISIS.E.RB2210016

ISIS Experiment Number: RB2210016

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr David Hardy et al; (2022): Tuneable-Membrane Protein Containing Floating Bilayers: Enhancement of Complexity Resolution through Differential Hydrogen Labelling, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210016

Data is released under the CC-BY-4.0 license.