ISIS Neutron and Muon Source Data Journal

Combined SANS, UV-visible and fluorescence to resolve assembly mechanisms in native silk

Abstract: Due to the chemical and structural complexities inherent to biological processes, the simultaneous combination of experimental techniques is necessary to gain reliable information about the mechanism and molecular basis for fibril/fibre assembly. A specific example is the role of reactive intermediates during the assembly of protein superstructures, such as in gelation, crystallisation, and fibrillation. Therefore, our aim, using the promising NUrF (Neutron small-angle scattering ? UV-visible - Fluorescence) platform at Larmor, is to uniquely extract the rate laws and resolve the intermediates (chemical and structural) involved in the formation of silk protein fibrils. This detailed and comprehensive characterization will allow for future rational design of assembly, with the final aim to mimic the extraordinary mechanical properties of silk in biomaterials.

Principal Investigator: Dr Cedric Dicko
Experimenter: Dr Judith Houston
Experimenter: Miss Juanita Francis
Experimenter: Dr Felix Roosen-Runge
Local Contact: Dr Robert Dalgliesh
Experimenter: Dr Andrew Jackson

DOI: 10.5286/ISIS.E.RB2210298

ISIS Experiment Number: RB2210298

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Cedric Dicko et al; (2022): Combined SANS, UV-visible and fluorescence to resolve assembly mechanisms in native silk, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210298

Data is released under the CC-BY-4.0 license.