ISIS Neutron and Muon Source Data Journal

Interaction of model phospholipid bilayer membranes with Extracellular vesicles

Abstract: Extracellular Vesicles (EVs)-mediated intercellular communication is recently receiving a rising attention. Owing to their content, EVs can target cargoes to promote beneficial or pathological conditions. However, understanding the mechanisms by which EVs interact with target cells is still a challenge. We address this by a multi-technique approach, using model membranes of different compositions. We isolated EVs from N2a cells in normal conditions and treated with the oligosaccharide OligoGM1, which is known for its neurotrophic/protective functions in the frame of Parkinson?s disease. Those 2 types of EVs were characterised by Western Blotting, DLS, NTA, AFM and DSC and have been found to have different chemico-physical characteristics. We propose here the use of NR for acquiring structural information on their detailed membrane profiles and interaction with target membrane models.

Local Contact: Dr Stephen Hall
Experimenter: Dr Alexandros Koutsioumpas
Experimenter: Dr Paola Brocca
Experimenter: Dr Valeria Rondelli
Experimenter: Dr Sally Mahmoud Helmy Abdalla

DOI: 10.5286/ISIS.E.RB2210198

ISIS Experiment Number: RB2210198

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Stephen Hall et al; (2022): Interaction of model phospholipid bilayer membranes with Extracellular vesicles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210198

Data is released under the CC-BY-4.0 license.