ISIS Neutron and Muon Source Data Journal

This is a page describing data taken during an experiment at the ISIS Neutron and Muon Source. Information about the ISIS Neutron and Muon Source can be found at https://www.isis.stfc.ac.uk.


Influence of pH on the triolein/aqueous interface and lipase activity

Abstract: Lipases are responsible for the degradation of triglycerides to smaller components and attract interest in industrial applications in both the food and pharmaceutical industries due to the catalytic behavior. Understanding the oil-water interface that is controlling lipolytic activity is therefore, of significant importance. In a recent study we investigated the influence of pH on the interfacial lipolysis of thin triolein films using ellipsometry. While these measurements provided information about reaction kinetics and changes in overall film thickness, no internal structural information was revealed. Here, we intend to use neutron reflectometry to probe how solvent pH influences the changes to the internal structure as well as the oil/solvent interface of a thin triolein film after exposure to an aqueous buffer solution and subsequent addition on a lypolytic enzyme.

Experimenter: Dr Ben Humphreys
Local Contact: Dr Mario Campana
Experimenter: Professor Tommy Nylander
Experimenter: Dr Thomas Arnold

DOI: 10.5286/ISIS.E.RB2210349

ISIS Experiment Number: RB2210349

Part DOI Instrument Public release date Download Link
10.5286/ISIS.E.RB2210349-1 INTER 16 March 2025 Download

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Ben Humphreys et al; (2022): Influence of pH on the triolein/aqueous interface and lipase activity, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2210349

Data is released under the CC-BY-4.0 license.



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