ISIS Neutron and Muon Source Data Journal

Impact of Protein Nanosheet Structure on Interfacial Viscoelasticity and Stem Cell Expansion

Abstract: Cell adhesion and proliferation at the surface of liquids such as oil droplets is a surprising phenomenon as it is typically accepted in the field of bioengineering that cells require solid substrates to adhere and to exert mechanical forces required for their spreading and proliferation. Our laboratory recently reported such observations and uncovered that such phenomenon was mediated by a nanoscale (15-20 nm) mechanically strong protein film (nanosheet) assembled at the interface between the two liquids. However, little is known of the morphology and structure of such protein nanosheets and the impact of such structures on their mechanics, and in turn cell behaviour. This project aims to explore the structure of protein nanosheets assembled at analogous oil-water interfaces, in situ.

Principal Investigator: Dr Ali Zarbakhsh
Local Contact: Dr Mario Campana
Experimenter: Professor Julien Gautrot

DOI: 10.5286/ISIS.E.RB2010050

ISIS Experiment Number: RB2010050

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Ali Zarbakhsh et al; (2021): Impact of Protein Nanosheet Structure on Interfacial Viscoelasticity and Stem Cell Expansion, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010050

Data is released under the CC-BY-4.0 license.