ISIS Neutron and Muon Source Data Journal

Interaction of RNA with titratable bilayers

Abstract: Lipid nanoparticles (LNPs) are a growing area of interest as drug delivery vehicles in mRNA therapies. Therapeutically relevant LNPs are complex, multicomponent systems with strongly pH dependent structure and interactions. Arteta et al. 2018 previously characterised an LNP system, which includes the cationic ionisable lipid DLin-MC3-DMA (MC3) and has shown therapeutic potential in vivo. The complex internal structure of these LNPs is poorly understood, therefore so is the interaction of MC3 with the mRNA cargo. To understand the complex system of Arteta et al, we propose to use a simpler system with the key components thought to control the RNA interaction. This consists of a two component lipid bilayer containing DOTAP (cationic lipid) or MC3 (titratable cationic lipid) and DOPC (phospholipid), which will allow us to reveal the effect of pH and charge density on adsorption of mRNA.

Principal Investigator: Professor Tommy Nylander
Experimenter: Dr Jennifer Gilbert
Experimenter: Dr Inna Ermilova
Local Contact: Dr Maxmilian Skoda
Experimenter: Professor Jan Swenson

DOI: 10.5286/ISIS.E.RB2010562

ISIS Experiment Number: RB2010562

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Tommy Nylander et al; (2021): Interaction of RNA with titratable bilayers, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010562

Data is released under the CC-BY-4.0 license.