ISIS Neutron and Muon Source Data Journal

SANS Study of interaction of Monoclonal Antibody with Non-ionic Surfactant

Abstract: Although we have made an extensive study of surface and interfacial adsorption of an engineered monoclonal antibody COE3, its solution structure has never been studied. As we know how COE3 is modified from the native IgG1, the proposed work here will lead to a better understanding of its conformation together with its constituting Fab and Fc in solution under different pHs. Then, we will examine how Fab, Fc and the whole mAb interact with modified Tween 80 with 7 and 13 EOs (PS80(7EO), PS80(13EO)). Preliminary studies have indicated that COE3 would interact with PS80(7EO) more favourably than the ones with longer EOs. Through appropriate contrast variations, SANS will help unravel the structural details relating to the binding of the nonionic surfactants to COE3 or its fragments. We will explore the new SASview based MD simulations for data analysis via support from the NIST team.

Principal Investigator: Professor Jian Lu
Local Contact: Dr James Doutch
Experimenter: Dr Peixun Li
Experimenter: Mr Peter Hollowell
Experimenter: Dr Zongyi Li

DOI: 10.5286/ISIS.E.RB2010727

ISIS Experiment Number: RB2010727

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2020): SANS Study of interaction of Monoclonal Antibody with Non-ionic Surfactant, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010727

Data is released under the CC-BY-4.0 license.