ISIS Neutron and Muon Source Data Journal

Elucidating the Membrane Insertion Mechanism of Chloride Intracellular Channel Proteins

Abstract: Chloride Intracellular Channels (CLICs) are a family of 6 proteins that have in common a C-terminal CLIC module and chloride-selective ion channel activity. CLIC1 is a 241 amino-acid protein involved in the regulation of the cell cycle, in particular it is overexpressed in a number of cancer cells. However, to date little information exists as to its structure and the factors that influence its insertion into membranes. Here we propose to supplement previous neutron reflectivity experiments with data collected using a deuterated form of the CLIC-1 protein. This will be used in combination with a broad range of ongoing biophysical characterisation to establish the mechanism of CLIC1 chloride channel insertion as a function of membrane charge. This will be an initial step to understand how CLIC1 functions in tumour cells and will allow specific treatments to be designed to interrupt it.

Principal Investigator: Dr Jose Luis Ortega Roldan
Experimenter: Miss Alex Hendry
Experimenter: Dr Rob Barker
Local Contact: Dr Mario Campana
Experimenter: Miss Chloe Marie Skingle
Local Contact: Dr Stephen Hall

DOI: 10.5286/ISIS.E.RB1920696

ISIS Experiment Number: RB1920696

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Jose Luis Ortega Roldan et al; (2021): Elucidating the Membrane Insertion Mechanism of Chloride Intracellular Channel Proteins, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920696

Data is released under the CC-BY-4.0 license.