ISIS Neutron and Muon Source Data Journal

How does oxidative damage change peptide membrane insertion?

Abstract: The insertion of peptides and proteins into cell membranes is crucial for the functioning of cells. Cells are continually subject to oxidation events. In this continuation experiment we will use neutron reflection to investigate how oxidative damage changes the interaction of the well-studied model peptide melittin with cell membrane models. In our previous work we observed changes in the membrane structure when oxidised lipids were present in the membrane, in this experiment we wish to extract more detailed structural information by using a deuterated form of the oxidized lipid, not previously available, that will allow us to clearly differentiate between the lipid and peptide components. The results will help us understand better the factors that influence the interaction of different peptides with cell membranes, and how that interaction is changed by oxidative damage.?

Principal Investigator: Dr Katherine Thompson
Local Contact: Dr Mario Campana
Experimenter: Professor Adrian Rennie
Experimenter: Mr Emil Gustafsson

DOI: 10.5286/ISIS.E.RB2010631

ISIS Experiment Number: RB2010631

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Katherine Thompson et al; (2020): How does oxidative damage change peptide membrane insertion? , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010631

Data is released under the CC-BY-4.0 license.