ISIS Neutron and Muon Source Data Journal

mRNA-lipoplexes for cancer immunotherapy

Abstract: mRNA-based nanomedicines show a broad field of potential applications, including tumor immunotherapy. One of these approaches consists of new ionizable lipids, which complex with charged RNA via electrostatical interactions, and can facilitate cellular uptake and endosomal escape of the RNA to the cytoplasm, while demonstrating orders-of-magnitude of efficacy. For more controlled development of such nanomedicines, a better understanding of the forming mechanisms for mRNA-lipoplexes is required. We propose an experiment where lipoplexes will be assembled from cationic liposomes and mRNA at different charge ratios. We then want to utilize the advantages of SANS, such as contrast variation, to better unterstand the internal structure of these delivery systems and distinguish between structural contributions from the different components. The data gained will correlated with biological data.

Principal Investigator: Professor Sir Peter Langguth
Local Contact: Dr Leide Cavalcanti
Experimenter: Mr Jorge Moreno Herrero
Experimenter: Dr ferdia bates
Experimenter: Mrs Vera Cornet
Experimenter: Dr Kaushik Thanki
Experimenter: Mr Lukas Uebbing
Experimenter: Dr Heinrich Haas

DOI: 10.5286/ISIS.E.RB2010649

ISIS Experiment Number: RB2010649

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Sir Peter Langguth et al; (2020): mRNA-lipoplexes for cancer immunotherapy, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010649

Data is released under the CC-BY-4.0 license.