ISIS Neutron and Muon Source Data Journal

Probing the kinetics of the formation of lipid, peptide and DNA/siRNA complexes intended for gene delivery

Abstract: Complexes prepared from cationic, dichain lipid (L) vesicles, positively charged polymers (P) and negatively charged nucleic acid DNA (D) or siRNA (R), known as LPDs and LPRs respectively have emerged as major non-viral gene delivery vehicles. Despite the wide spread interest in such complexes as delivery vehicles, little is known about the kinetics of their formation. The present study proposes to use stopped flow SANS measurements in combination with contrast variation to probe the mechanism of formation of the LPD and LPR complexes. An understanding of the mechanisms behind the formation of LPDs and LPRs will allow the production of more homogeneous, efficient delivery systems in a pharmaceutically acceptable form.

Principal Investigator: Professor Jayne Lawrence
Experimenter: Dr Yu Lu
Local Contact: Dr Diego Alba Venero

DOI: 10.5286/ISIS.E.RB2010694

ISIS Experiment Number: RB2010694

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2020): Probing the kinetics of the formation of lipid, peptide and DNA/siRNA complexes intended for gene delivery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010694

Data is released under the CC-BY-4.0 license.