ISIS Neutron and Muon Source Data Journal

Unravelling the distinct biological activities of the four industrially- and medically-important human IgG1-IgG4 antibody subclasses

Abstract: The different antibody hinges at the centre of the human IgG1, IgG2, IgG3 and IgG4 subclasses are key to their very different reactivities, such as binding to their Fc receptors. A molecular understanding of these marked molecular differences between the subclasses is key for understanding major applications of the human antibodies as pharmaceuticals, as well as the role of IgG in disease. We have an extensive background in the atomistic modelling of IgG1, IgG2 and IgG4 antibodies. We have obtained very clean preparations of polyclonal and myeloma human IgG3, and have high quality X-ray scattering data on this. By performing neutron scattering of these IgG3 and their deglycosylated forms, and submitting the scattering curves to atomistic modelling, we will identify the solution structures of this important subclass and how its stability is affected by glycans.

Principal Investigator: Professor Stephen Perkins
Experimenter: Miss Hina Iqbal
Local Contact: Dr James Doutch
Experimenter: Miss Valentina Spiteri

DOI: 10.5286/ISIS.E.RB2010735

ISIS Experiment Number: RB2010735

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Stephen Perkins et al; (2020): Unravelling the distinct biological activities of the four industrially- and medically-important human IgG1-IgG4 antibody subclasses , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010735

Data is released under the CC-BY-4.0 license.