ISIS Neutron and Muon Source Data Journal

Fluorescent Labelling of Viruses for Rapid Optical Identification of Viral Pathogens

Abstract: Infectious diseases caused by viruses such as influenza, HIV, Ebola, measles, Zika and hepatitis represent a huge global public health concern, causing many thousands of deaths annually. During the last two years we have worked on developing methods for the rapid fluorescent labelling of viruses, with the aim of developing an optical biosensor for virus detection based on fluorescent ribonucleic acid binding agents. We aim to use neutron reflectometry to characterise the exact mechanism of fluorescence labelling on the membrane surface of enveloped virus particles. Our experiments to date suggest that efficient fluorescence labelling of enveloped viruses requires the presence of a divalent cations fluorescently-labelled nucleic acid and anionic lipid bilayers on the virus particle. We will model this interaction using supported lipid bilayers to gain precision molecular information.

Principal Investigator: Dr Nicole Robb
Local Contact: Dr Luke Clifton
Experimenter: Mr Nicolas Shiaelis

DOI: 10.5286/ISIS.E.RB2010773

ISIS Experiment Number: RB2010773

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Nicole Robb et al; (2021): Fluorescent Labelling of Viruses for Rapid Optical Identification of Viral Pathogens, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010773

Data is released under the CC-BY-4.0 license.