ISIS Neutron and Muon Source Data Journal

Lipid Interactions with Styrene-Maleic Acid Copolymers on Surfaces

Abstract: Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied discs formed using a poly(styrene-alt-maleic acid) (PSMA) copolymers synthesised using RAFT controlled radical polymerisations. The RAFT method is a living polymerisation allowing us to grow either an alternating styrene (S) and maleic acid (MA) segment first or a pure styrene section. We have tethered the RAFT agent to oxide surfaces allowing us to grow the polymer from the interface, and have demonstrated that the surface tethered polymer interacts strongly with lipid vesicles, pulling them out of suspension. Here we wish to use neutron reflectivity with contrast variation to determine the surface structures which develop in this system, and how these vary with the position of the S-MA vs S blocks relative to the surface.

Principal Investigator: Professor Karen Edler
Experimenter: Ms Kerrie Morrison
Experimenter: Dr Thomas Arnold
Experimenter: Mr George Neville
Local Contact: Dr Mario Campana
Experimenter: Dr Iva Manasi
Experimenter: Dr James Doutch

DOI: 10.5286/ISIS.E.RB1920425

ISIS Experiment Number: RB1920425

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2020): Lipid Interactions with Styrene-Maleic Acid Copolymers on Surfaces, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920425

Data is released under the CC-BY-4.0 license.