ISIS Neutron and Muon Source Data Journal

Structure of mixed lipid monolayers containing bacterial lipoteichoic acid (LTA) at the air-water interface

Abstract: Lipoteichoic acid (LTA), a polymeric amphiphile with an interesting architecture (lipids+polymer brushes) present in the Gram-positive bacterial membrane, is considered a major cause of various infections. The fundamental understanding on LTA-induced structural changes to the phospholipid membrane remains inadequate. We propose to investigate the structure of the mixed lipid monolayers mimicking the outer lipid membrane structure of Bacillus subtilis, a Gram-positive bacteria species comprising d-DPPG, d-DPPE, CL and LTA using neutron reflectivity (NR) at the air-water interface, particularly varying the LTA content (2-15 mole %). The subphase pH and temperature will also be varied to understand their effects on the structural integrity of the mixed lipid monolayer mimicking the outer leaflet of the bacterial membrane.

Principal Investigator: Professor Wuge Briscoe
Experimenter: Miss Anna Slastanova
Experimenter: Mr Michael Stevens
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Bhaveshkumar Narendrabhai Bharatiya
Experimenter: Miss Lauren Matthews
Experimenter: Miss Charlotte Kenton
Experimenter: Miss Xueying Guo
Experimenter: Miss Holly Stockdale
Experimenter: Mr Gang Wang
Local Contact: Dr Mario Campana

DOI: 10.5286/ISIS.E.RB2010222

ISIS Experiment Number: RB2010222

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Wuge Briscoe et al; (2021): Structure of mixed lipid monolayers containing bacterial lipoteichoic acid (LTA) at the air-water interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010222

Data is released under the CC-BY-4.0 license.