ISIS Neutron and Muon Source Data Journal

The Interactions of Blood Components with Models of the Gram Negative Bacterial Surface

Abstract: There is a lack of understanding in how medicines interact with blood serum components and with either bacterial or endothelial cellular membranes. We propose to examine the interaction of high density lipoprotein (HDL) and free apolipoproteins (ApoA1) with differing supported membrane types at the silicon water interface. One, a model of the eukaryotic membrane composed of mixed DMPCDMPS-Cholesterol 8:1:1 and another an asymmetric model of the Gram negative bacterial outer surface. We will systematically test our hypothesis that the LPS in the distal leaflet of the Gram negative bacterial outer membrane prevents serum component interactions with the bacterial surface and thus defends the bacterium from all but highly specific (anti-LOS antibodies) serum component interactions, and we will be able to determine the effect of cholesterol and Calcium on lipid exchange.

Principal Investigator: Professor Marité Cárdenas Gómez
Experimenter: Miss Sarah Waldie
Local Contact: Dr Luke Clifton
Experimenter: Dr Nicolò Paracini

DOI: 10.5286/ISIS.E.RB2010739

ISIS Experiment Number: RB2010739

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Marité Cárdenas Gómez et al; (2020): The Interactions of Blood Components with Models of the Gram Negative Bacterial Surface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010739

Data is released under the CC-BY-4.0 license.