ISIS Neutron and Muon Source Data Journal

SEC-SANS for structure determination for membrane proteins in polymer-stabilised nanodiscs

Abstract: This proposal is a continuation from our upcoming experiment aimed at using Size-Exclusion Chromatography coupled with SANS (SEC-SANS) to ensure accurate measurement of membrane proteins supported in Poly(Styrene-Maleic Acid) (SMA) lipid-nanodiscs. These polymer-formed nanodiscs are an appealing choice compared to more traditional alternatives for protein extraction and structural studies, as preparations are stable, quick, low cost and can be produced in large quantities. In this proposal we propose to use again the membrane proteins gramicidin and REAMP within polymer-nanodiscs benchmarked in our upcoming experiment where polymer variant, concentration and deuteration conditions will be optimised. REAMP is a de novo membrane protein containing four alpha-helical transmembrane domains while gramicidin is readily available to facilitate initial scoping experiments using SEC-SANS.

Principal Investigator: Professor Karen Edler
Local Contact: Dr James Doutch
Experimenter: Ms Kerrie Morrison
Experimenter: Dr Luke Clifton
Experimenter: Mr George Neville
Experimenter: Dr Marcelo da Silva
Experimenter: Dr Nathan Cowieson
Experimenter: Mr Nikul Khunti
Experimenter: Dr Charlotte Jennifer Chante Edwards-Gayle
Local Contact: Dr Najet Mahmoudi

DOI: 10.5286/ISIS.E.RB2010211

ISIS Experiment Number: RB2010211

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2021): SEC-SANS for structure determination for membrane proteins in polymer-stabilised nanodiscs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2010211

Data is released under the CC-BY-4.0 license.