ISIS Neutron and Muon Source Data Journal

Electrostatic Control of Protein Adsorption under Re-entrant Conditions

Abstract: Protein adsorption is a process relevant in many research fields such as biology, medicine and food processing. Its mechanism is influenced by many parameters. Our group has demonstrated that strongly directional interactions induced by ion-bridges connecting individual proteins give rise to a rich phase behaviour including re-entrant condensation and liquid-liquid phase separation (LLPS). Other findings suggest that potential applied to a substrate is able to polarise the adsorbed layer and induce dipole–dipole interactions between the adsorbed and the incoming protein. Building on our recent discovery of ‘re-entrant’ adsorption, we would like to investigate how the surface potential influences the structure (and kinetics) of the adsorption of BSA solutions at high concentrations and in the presence of multivalent ions.

Principal Investigator: Dr Maxmilian Skoda
Experimenter: Professor Frank Schreiber
Local Contact: Dr Andrew Caruana
Experimenter: Ms Madeleine Fries
Experimenter: Dr Robert Jacobs
Experimenter: Ms Nina Conzelmann

DOI: 10.5286/ISIS.E.RB1910571

ISIS Experiment Number: RB1910571

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Maxmilian Skoda et al; (2020): Electrostatic Control of Protein Adsorption under Re-entrant Conditions, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910571

Data is released under the CC-BY-4.0 license.