ISIS Neutron and Muon Source Data Journal

The Molecular Architecture of Cationic Lipid Nanoparticles for siRNA Delivery

Abstract: The proposal aims to use SANS and contrast variation to determine the detailed molecular architecture of lipid nanoparticles (LNPs) containing the nucleic acid, siRNA. The information thus gained will aid an understanding of the relationship between the cationic lipids used to prepare the LNP, the structure of the LNP they form and the LNP's therapeutic activity. Recently, work in our group has shown that the preparation of LNPs using trichain cationic lipids results in better siRNA delivery than when using the more usual dichain cationic lipids. The current proposal will therefore use SANS to compare the molecular architecture of LNPs prepared using trichain cationic lipids with that seen with the corresponding dichain lipids and relate this to the ability of the LNPs to deliver siRNA. The work is important for the development of improved nucleic acid delivery vehicles as medicines.

Principal Investigator: Professor Jayne Lawrence
Local Contact: Dr Najet Mahmoudi
Experimenter: Dr Dave Barlow
Experimenter: Dr Alex Clout
Experimenter: Dr Alice Spadea
Experimenter: Dr Yu Lu
Experimenter: Miss Yixuan Yan

DOI: 10.5286/ISIS.E.RB1920449

ISIS Experiment Number: RB1920449

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
Select the data format above to find out more about it.

Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2020): The Molecular Architecture of Cationic Lipid Nanoparticles for siRNA Delivery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920449

Data is released under the CC-BY-4.0 license.