ISIS Neutron and Muon Source Data Journal

Effect of formulation method and lipid composition on liposome lamellarity

Abstract: Liposomes are vesicles comprising a phospholipid membrane bilayer around an aqueous core. They are well-established in the fields of drug delivery and diagnostics. To translate formulations from bench to bedside, their structure and morphology must be precisely understood. Specifically, characterising lamellarity (number of bilayers) in vesicle populations is crucial as it influences release kinetics and encapsulation efficiency. We have characterised the effect of formulation method and lipid composition on vesicle lamellarity in phosphocholine vesicles. Here, we propose applying these models to study phospholipid-based vesicles which more closely represent those in pharmaceutical formulations, which include cholesterol, and positively and negatively charged lipids. The results are highly significant when designing vesicle formulations for applications in diagnostics and drug delivery.

Principal Investigator: Dr Margaret Holme
Experimenter: Dr Michael Thomas
Experimenter: Miss Valeria Nele
Experimenter: Dr Hanna Barriga
Local Contact: Dr James Doutch
Experimenter: Professor Molly Stevens
Experimenter: Dr Jelle Penders
Experimenter: Miss Catherine Saunders
Experimenter: Miss Nayoung Kim
Experimenter: Mr Michael Potter

DOI: 10.5286/ISIS.E.RB1920464

ISIS Experiment Number: RB1920464

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Margaret Holme et al; (2020): Effect of formulation method and lipid composition on liposome lamellarity, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920464

Data is released under the CC-BY-4.0 license.