ISIS Neutron and Muon Source Data Journal

Solubilisation of membrane protein REAMP in polymer-stabilised nanodiscs

Abstract: Poly(Styrene-Maleic Acid) (SMA) is increasingly used in the formation of lipid-nanodiscs for structural membrane studies and biophysics. These polymer-formed nanodiscs are an appealing choice compared to more traditional alternatives, as preparations are stable, quick, low cost and can be produced in large quantities. In this proposal we wish to study the membrane proteins gramicidin and REAMP within polymer-nanodiscs using SMA variants. REAMP is a de novo membrane protein containing four alpha-helical transmembrane domains while gramicidin is readily available. This study will enable us to evaluate the key contrasts required for membrane protein study when incorporated into nanodiscs and whether REAMP maintains its native formation, ultimately to obtain its 3D structure. This highlights the potential to use polymer-stabilised nanodiscs for membrane protein structural analysis using SANS

Principal Investigator: Professor Karen Edler
Experimenter: Dr James Doutch
Experimenter: Mr George Neville
Local Contact: Dr Leide Cavalcanti
Experimenter: Ms Kerrie Morrison
Experimenter: Dr Marcelo da Silva
Experimenter: Dr Luke Clifton

DOI: 10.5286/ISIS.E.RB1920099

ISIS Experiment Number: RB1920099

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2020): Solubilisation of membrane protein REAMP in polymer-stabilised nanodiscs, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920099

Data is released under the CC-BY-4.0 license.