ISIS Neutron and Muon Source Data Journal

Lipid nanoparticles-Apolipoprotein E interaction: the role of LNP surface composition and structure

Abstract: Therapeutic treatments based on the production of proteins by delivering messenger RNA (mRNA) represent a promising approach. Lipid nanoparticles (LNPs) formed by a cationic ionizable lipid (CIL), DSPC, cholesterol and a pegylated lipid have been successful to deliver small interference RNA. The bio-distribution and cellular uptake of LNPs are affected by their surface composition as well as by the extracellular proteins present at the site of LNPs administration, e.g. ApolipoproteinE (ApoE). ApoE plays a key role in the LNP’s circulation time. Our previous results show that both particle size and DSPC surface area affects the efficacy of LNPs. For an optimised LNP formulation, we aim to reveal the contribution of CIL and cholesterol to the LNP structure. Furthermore, we want to study the role of LNP surface structure on the ApoE binding and the structural change due to ApoE binding.

Principal Investigator: Dr Federica Sebastiani
Experimenter: Professor Marité Cárdenas Gómez
Local Contact: Dr Najet Mahmoudi

DOI: 10.5286/ISIS.E.RB1920068

ISIS Experiment Number: RB1920068

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Federica Sebastiani et al; (2020): Lipid nanoparticles-Apolipoprotein E interaction: the role of LNP surface composition and structure, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920068

Data is released under the CC-BY-4.0 license.