ISIS Neutron and Muon Source Data Journal

SANS investigation of proteins trapped in hydrogels formed by binary mixtures of G/GMP in water

Abstract: Aqueous solutions of guanosine 5’-monophosphate (GMP) can turn into isotropic gels by the addition of guanine (Gua). The Gua:GMP hydrogel property of including a significant amount of water opens a series of applications in the field of biocompatible nanomaterials, including the trapping of proteins and enzymes. Recently, we have investigated the properties of Gua:GMP hydrogels, with and without trapped bovine serum albumin or myoglobin by using AFM and micro DSC techniques. Depending on composition, proteins can be trapped as monomers, in the large water domains of the gel, or as protein clusters, located at the junction points of the gel fibres. On this basis, we intend to in-deep investigate with SANS structure and aggregation state of proteins trapped in the hydrogel. Experiments will be performed at the optimum matching conditions between D2O/H2O and hydrogel

Principal Investigator: Professor Francesco Spinozzi
Experimenter: Professor Paolo Mariani
Experimenter: Dr Astra Piccinini
Experimenter: Miss Federica Carducci
Local Contact: Dr James Doutch
Experimenter: Miss Alessia Pepe
Experimenter: Mrs Alessia Belloni [Deactivated]

DOI: 10.5286/ISIS.E.RB1920325

ISIS Experiment Number: RB1920325

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Francesco Spinozzi et al; (2019): SANS investigation of proteins trapped in hydrogels formed by binary mixtures of G/GMP in water, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920325

Data is released under the CC-BY-4.0 license.