ISIS Neutron and Muon Source Data Journal

The membrane lipid composition regulates the orientation of Tissue Factor extramembrane domain

Abstract: Tissue Factor (TF) is a membrane protein located on the cell surfaces of extravascular cells. TF activity is fundamental for normal homeostasis- the physiological process which leads to blood coagulation. Indirect evidences suggest that the membrane lipid composition and in particular the presence of anionic phospholipid, such as phosphatidylserine (PS), and cholesterol has an important impact in stabilizing the protein complex and thus in regulating TF activity towards blood coagulation. No experimental data has so far been published on the structure of TF in lipid membranes. Here we propose to prepare supported membrane with TF to investigate by means of NR the effect of PS and cholesterol on the TF location with respect to the membrane.

Principal Investigator: Dr Alessandra Luchini
Experimenter: Dr Raul Araya Secchi
Experimenter: Professor Lise Arleth
Local Contact: Dr Mario Campana
Experimenter: Mr Frederik Tidemand
Experimenter: Mr Nicolai Johansen

DOI: 10.5286/ISIS.E.RB1920320

ISIS Experiment Number: RB1920320

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Alessandra Luchini et al; (2019): The membrane lipid composition regulates the orientation of Tissue Factor extramembrane domain, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920320

Data is released under the CC-BY-4.0 license.