ISIS Neutron and Muon Source Data Journal

Human telomeric G-quadruplex complexed with the highly selective anticancer drug BRACO-19. A SANS investigation along the melting path.

Abstract: G-quadruplexes (G4) are the focus of a number of studies in both fundamental and applied research, from cancer biology and novel therapeutics through to nanotechnology. The G4 topology depends on a variety of factors, such as the temperature and the specific interaction with ligands, i.e. potential anti-cancer drugs. Quite recently we have shown that in the case of the human telomeric quadruplex Tel22 both the free system and the one complexed with a model drug undergo melting through a multi-state process. This information is crucial to correctly modelize the Tel22 stability. Here we ask to do a SANS investigation of the complexation of Tel22 with BRACO-19, a potent stabilizer ligand with a remarkable in vivo single-agent antitumor activity. SANS measurements along the thermal unfolding path will reveal the large-scale structural features of the native and intermediate states.

Principal Investigator: Professor alessandro paciaroni
Experimenter: Dr Lucia Comez
Local Contact: Dr Najet Mahmoudi
Experimenter: Professor Francesco Spinozzi

DOI: 10.5286/ISIS.E.RB1920368

ISIS Experiment Number: RB1920368

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor alessandro paciaroni et al; (2019): Human telomeric G-quadruplex complexed with the highly selective anticancer drug BRACO-19. A SANS investigation along the melting path., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920368

Data is released under the CC-BY-4.0 license.