ISIS Neutron and Muon Source Data Journal

Membrane reconstitution of human dihydroorotate dehydrogenase using lipid-detergent micelles

Abstract: Human dihydroorotate dehydrogenase (DHODH) is an integral membrane protein found in mitochondria involved in the biosynthesis of pyrimidines. DHODH oxidizes dihydroorotate with the simultaneous reduction of ubiquinone Q10 and is a well-validated target for anti-inflammatory and anti-proliferative drugs. We are investigating the mechanisms by which DHODH interacts with Q10 and clinical inhibitors in the inner mitochondrial membrane (IMM) and therefore develop methods to reconstitute it into physiologically lipid bilayers mimicking the IMM using phospholipid-detergent micelles. In this experiment, we propose will investigate for the first time the reconstitution of DHODH into supported bilayers from phospholipid-dodecyl-D-maltoside (DDM) micelles using contrasts variation to determine the composition and structure of the membrane during the deposition process.

Principal Investigator: Dr Wolfgang Knecht
Local Contact: Dr Luke Clifton
Experimenter: Dr Hanna Wacklin-Knecht
Experimenter: Mr Juan Manuel Orozco Rodriguez

DOI: 10.5286/ISIS.E.RB1920176

ISIS Experiment Number: RB1920176

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Wolfgang Knecht et al; (2019): Membrane reconstitution of human dihydroorotate dehydrogenase using lipid-detergent micelles, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920176

Data is released under the CC-BY-4.0 license.