ISIS Neutron and Muon Source Data Journal

Neutron scattering and selective deuteration for elucidating how lipids regulate metabolon formation

Abstract: Plants biosynthesise valuable pharmaceutical compounds by a series of enzyme complexes (or metabolons) that work in a concerted action. Metabolons enhance substrate channeling and prevent leakage of toxic or labile intermediates and metabolic cross-talk. Extracting pure products from plats constitutes a great industrial and societal challenge, and bioengineering of moss and bacteria was come up as a very interesting way to produce these compounds. Here, we will dissect the mechanisms by which plants guide the assembly of multi-enzyme complexes. We will use synthetic biology and neutron scattering to map the lipid-protein interactions that control the formation of the dhurrin metabolon, as a model system. This will enable rational enzyme design for metabolons in heterologous hosts to boost the production of bioactive compounds. This experiment will provide the first basis for such studies

Principal Investigator: Professor Marité Cárdenas Gómez
Local Contact: Dr James Doutch
Experimenter: Dr Tomas Laursen
Experimenter: Dr Rita Del Giudice
Experimenter: Dr Christopher Garvey
Experimenter: Mr Artur Glavic
Experimenter: Dr Nicolò Paracini
Experimenter: Professor Max Wolff

DOI: 10.5286/ISIS.E.RB1920128

ISIS Experiment Number: RB1920128

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Marité Cárdenas Gómez et al; (2019): Neutron scattering and selective deuteration for elucidating how lipids regulate metabolon formation, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1920128

Data is released under the CC-BY-4.0 license.