ISIS Neutron and Muon Source Data Journal

RESUBMISSION : DNA hybridisation-mediated vesicle fusion - kinetics and fate of fusion

Abstract: Controlled vesicle fusion has major advantages in diagnostic and drug delivery applications. We have designed a DNA hybridisation-based assay for directed fusion of vesicles upon mixing, and applied it in a recently published diagnostic assay to detect microRNA. In this proposal we propose static and stopped flow SANS experiments with contrast matching to study this DNA-mediated vesicle fusion mechanism and gain insight into 1) changes to vesicle shape in real time during fusion, and 2) mixing of the inner and outer membrane leaflets. We will confirm if DNA-functionalised liposomes proceed to hemi- or fully fused states, and if fusion can be controlled by modifying vesicle lipid composition and DNA concentration. The findings are essential to understand these vesicles fusion behaviour and can be directly applied to developing drug delivery vectors and diagnostic assays within our lab.

Principal Investigator: Dr Margaret Holme
Local Contact: Dr James Doutch
Experimenter: Dr Hanna Barriga
Experimenter: Dr E Kim
Experimenter: Dr Michael Thomas
Experimenter: Professor Molly Stevens
Experimenter: Dr Christopher Wood
Experimenter: Mr Brian Chen
Experimenter: Dr Omar Rifaie Graham
Experimenter: Dr Adrian Najer
Experimenter: Mr Michael Potter
Experimenter: Miss Nayoung Kim
Experimenter: Dr Adam Creamer

DOI: 10.5286/ISIS.E.RB1900140

ISIS Experiment Number: RB1900140

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Margaret Holme et al; (2019): RESUBMISSION : DNA hybridisation-mediated vesicle fusion - kinetics and fate of fusion, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1900140

Data is released under the CC-BY-4.0 license.