ISIS Neutron and Muon Source Data Journal

Effect of Phosphatidylserine lipids on Tissue Factor structure and function in lipid membranes

Abstract: Tissue Factor (TF) is the membrane protein responsible for the initiation of the blood coagulation process [1]. In case of a vessel injury, the TF is exposed to the blood stream and initiates a series of cascade reactions the first of which being the formation of a protein complex with the coagulation factors FVIIa (TF/FVIIa) and FXa (TF/FVIIa/FXa). No experimental data has so far been published on the structure of TF or TF/FVIIa/FXa in lipid membranes. Recently we developed a new protocol for fast and robust preparation of lipid bilayers with membrane proteins, which we propose here to use for investigating TF. The proposed experiment will provide the needed data to finalise a manuscript already in preparation. A PhD student is involved in this project; this additional experiment will provide fundamental experimental data for his thesis (thesis submission deadline in September).

Principal Investigator: Dr Alessandra Luchini
Experimenter: Mr Emmanouil Terzidis
Experimenter: Miss Chastine Munk
Experimenter: Dr Raul Araya Secchi
Local Contact: Dr Mario Campana
Experimenter: Professor Lise Arleth
Experimenter: Mr Frederik Tidemand

DOI: 10.5286/ISIS.E.RB2000012

ISIS Experiment Number: RB2000012

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Alessandra Luchini et al; (2019): Effect of Phosphatidylserine lipids on Tissue Factor structure and function in lipid membranes, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB2000012

Data is released under the CC-BY-4.0 license.