ISIS Neutron and Muon Source Data Journal

Cryopreservation of liver cell spheroids used in artificial liver bio-transplant: diffraction study.

Abstract: The development of new cryopreservation strategies has major potential in medicine, in particular for storage of biological matter. The detailed characteristics of the cooling and thawing processes has proven crucial for the survival of liver bio-transplant cells in research being done at UCL's Royal Free Hospital Liver Group. A specially designed protocol ensures that more than 75% of cells survive cooling followed by thawing. Currently there is no clear explanation as to why this happens, the hypothesis being that it relates to the formation of glass pockets within an ice matrix. Previous measurement at ISIS have allowed us to prove the existence of a larger glass to ice ratio with the special protocol that when quench. We ask to continue the study by a detailed structural (diffraction) and dynamical (QENS) characterisations of the ice formation and the glassy pockets respectively.

Principal Investigator: Professor Barry Fuller
Experimenter: Mr Maooz Awan
Experimenter: Dr Oleg Kirichek
Local Contact: Dr Dominic Fortes
Experimenter: Professor clare selden
Experimenter: Dr Victoria Garcia Sakai

DOI: 10.5286/ISIS.E.RB1910591

ISIS Experiment Number: RB1910591

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Barry Fuller et al; (2019): Cryopreservation of liver cell spheroids used in artificial liver bio-transplant: diffraction study., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1910591

Data is released under the CC-BY-4.0 license.