ISIS Neutron and Muon Source Data Journal

Quantifying the Asymmetry in Cell Membrane Mimetic Supported Lipid Bilayers.

Abstract: Cell membranes are characterised by complex lipid composition and asymmetric distribution of lipids (lipids facing the outside of the cell are different from the lipids that are facing the inside of the cell). We developed a model system that captures these features. It is based on TiO2-supported four-component lipid bilayers containing the canonical mixture of high-melting and low-melting phosphatidylcholines + cholesterol, plus phosphatidyl serine (PS). PS strongly interacts with TiO2, leading to its asymmetric distribution and mimicking the cytoskeleton-cell membrane interactions. The goal of this proposal is to quantify the distribution of all lipid components in these asymmetric cell membrane mimics by neutron reflectometry. Additional insight is gained by comparing it to that in membranes prepared on SiO2 from the same liposomes.

Principal Investigator: Dr Ilya Reviakine
Local Contact: Dr Maxmilian Skoda
Experimenter: Dr Rob Barker

DOI: 10.5286/ISIS.E.RB1520054

ISIS Experiment Number: RB1520054

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Ilya Reviakine et al; (2015): Quantifying the Asymmetry in Cell Membrane Mimetic Supported Lipid Bilayers., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520054

Data is released under the CC-BY-4.0 license.