ISIS Neutron and Muon Source Data Journal

NR study of surface adsorption of antibodies with and without non-ionic surfactants

Abstract: Nonionic surfactants usually do not show affinity to proteins and they work via competitive surface and interfacial processes in abinary mixture. However, we show in this work that Tween 80, a much bigger nonionic molecule than C12E5, with an oleyl chain, could initiate interaction possibly via the hydrophobic effect, evident from the surface tension plots. We propose to examine their surface co-adsorption processes by neutron reflection to gain direct structural evidence of the interaction, as this is a rather new observation from such systems. We request 3 days of Inter beam time to exploit the contrast variations by using deuterated Tween 80 in combination with 3 different water contrasts (weak signals under some contrast combinations). The proposed measurements will enable us to analyse the structures via the kinematic approach.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Chris van der Walle
Experimenter: Dr Lisa Pan
Experimenter: Dr John Webster
Experimenter: Mr Robert Holman
Experimenter: Dr Peixun Li
Local Contact: Dr Mario Campana
Experimenter: Mr Charles Smith

DOI: 10.5286/ISIS.E.RB1520328

ISIS Experiment Number: RB1520328

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2015): NR study of surface adsorption of antibodies with and without non-ionic surfactants, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520328

Data is released under the CC-BY-4.0 license.