ISIS Neutron and Muon Source Data Journal

Membrane perturbation and permeabilization by the lysosomotropic detergent MSDH

Abstract: Lysosomes are subcellular organelles with acidic interiors (pH 3.5-5), containing enzymes that break down macromolecules and intracellular waste. Controlled release of lysosomal contents incurs programmed cell death (apoptosis), and may be regulated by lysosomotropic detergents (LDs), which are weakly basic amphiphiles. We study the synthetic LD O-methyl-serine dodecylamide hydrochloride (MSDH), in both model membranes and in cells, to clarify the permabilization mechanism, for which the pH difference between lysosome and cytosol is believed to be important. Cell studies show that MSDH cause apoptosis through lysosomal membrane permeabilization. A strong pH-dependence on MSDH aggregation and interaction with lipids has been confirmed by DLS and vesicle leakage assays. We ask for beamtime to investigate the the effects of MSDH on model lysosomal membranes under different conditions.

Principal Investigator: Dr Thomas Ederth
Local Contact: Dr Sarah Rogers
Experimenter: Dr Timmy Fyrner
Experimenter: Dr Ana Villamil Giraldo
Experimenter: Professor Karin Öllinger

DOI: 10.5286/ISIS.E.RB1520420

ISIS Experiment Number: RB1520420

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Thomas Ederth et al; (2015): Membrane perturbation and permeabilization by the lysosomotropic detergent MSDH, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520420

Data is released under the CC-BY-4.0 license.