ISIS Neutron and Muon Source Data Journal

A complex model of the bacterial outer membrane containing oriented proteins and smooth LPS.

Abstract: The Outer Membrane (OM) is found in bacteria which cause plague, cholera & Legionnaire?s disease. It is important as; a barrier to antibiotics & the site of human-bacteria interaction. However bacteria are too small to allow us to do precise measurements of the structure and dynamics of this critical interface. Using neutrons as our eyes we have recently investigated OM models which imitate many aspects of the natural OM. This includes its structure where the outer surface is dominated by a molecule called lipopolysaccharide (LPS). However the model is unstable if we use the very large versions of LPS found in some bacteria. This project aims to understand this by fixing the other components of the model to a surface and just allowing the LPS to assemble and disassemble. In this way we seek to understand what maintains the structure of the natural OM and thus improve our future models.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Anton Le Brun
Experimenter: Dr Stephen Holt
Experimenter: Dr Luke Clifton
Experimenter: Dr Nicolò Paracini
Local Contact: Dr Timothy Charlton

DOI: 10.5286/ISIS.E.RB1520224

ISIS Experiment Number: RB1520224

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2015): A complex model of the bacterial outer membrane containing oriented proteins and smooth LPS., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520224

Data is released under the CC-BY-4.0 license.