ISIS Neutron and Muon Source Data Journal

Polymer-Lipid Nanodisc Self-assembly Processes Using Stop Flow

Abstract: Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Polymer stabilized lipid bilayer discs are easily made by adding polymer to a suspension of lipids or disrupted cell membranes, with discs forming within ~5 minutes. It is not clear how the polymer forms discs from these systems, since in vesicles or cell membranes the headgroups are exposed to solution, and the soluble polymer, but in the final discs, the polymer is wrapped around the tail regions of the lipid bilayer. We have also observed that nanodiscs are dynamic, and undergo exchange of lipids between discs or between discs and lipid bilayers on ~10 min timescales. Here we propose to use stop-flow on Sans2d to study nanodisc formation from a solution of vesicles mixed with polymer, and also to probe the kinetics of lipid exchange between nanodiscs.

Principal Investigator: Professor Karen Edler
Experimenter: Dr Ann Terry
Experimenter: Ms Cecilia Tognoloni
Experimenter: Dr Thomas Arnold

DOI: 10.5286/ISIS.E.RB1510436

ISIS Experiment Number: RB1510436

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2015): Polymer-Lipid Nanodisc Self-assembly Processes Using Stop Flow, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1510436

Data is released under the CC-BY-4.0 license.