ISIS Neutron and Muon Source Data Journal

Effect of oxidation on lipid raft formation: an off-specular neutron reflectivity study

Abstract: The very heterogeneous, but non-random, arrangement of lipids and proteins in cell membranes, known as rafts are of major scientific interest. Lipids in cell membranes are continually under attack from reactive oxygen species with the result that new, oxidized species of lipid are continually being formed. Although oxidized lipids have implications in cell apoptosis, cancer and aging, very little work has been performed to understand the effect of oxidative lipid damage on raft formation. We propose, therefore, to use specular and off-specular neutron reflection in combination with contrast variation to probe the effect of the presence of oxidized lipid on the persistence and size of rafts formed. These studies will allow a better understanding of the factors leading to the formation of microdomains in biological membranes and in particular the influence of oxidation thereupon.

Principal Investigator: Professor Jayne Lawrence
Experimenter: Dr Robert Dalgliesh
Experimenter: Dr Ivana Knyght
Experimenter: Dr Katherine Thompson

DOI: 10.5286/ISIS.E.RB1510424

ISIS Experiment Number: RB1510424

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jayne Lawrence et al; (2015): Effect of oxidation on lipid raft formation: an off-specular neutron reflectivity study, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1510424

Data is released under the CC-BY-4.0 license.