ISIS Neutron and Muon Source Data Journal

Structural studies of Staufen1 in complex with Upf1, a crucial assembly in gene regulation

Abstract: Gene expression is regulated during both the synthesis and the decay of mRNAs. Various pathways of cytoplasmic mRNA decay exist. These include ARE-mediated decay, miRNA-Mediated mRNA decay, Nonsense-Mediated mRNA decay (NMD), and the recently discovered Staufen-Mediated mRNA decay (SMD) (1). We want to understand the interplay of Staufen1 (Stau1), a dsRNA-binding protein, with a key mediator of mRNA decay, the Upf1 helicase, as they come together on an mRNA substrate. To do so, we will integrate small angle scattering, biophysical and biochemical analyses with electron microscopy studies. The proposed work will provide unique mechanistic insights into an important and medically relevant mechanism of gene expression regulation in humans.

Principal Investigator: Dr Laura Spagnolo
Experimenter: Mr Dmytro Kompaniiets
Experimenter: Dr Daniel Myatt
Experimenter: Mr Giuseppe Cannone
Local Contact: Dr Sarah Rogers

DOI: 10.5286/ISIS.E.RB1510371

ISIS Experiment Number: RB1510371

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Laura Spagnolo et al; (2015): Structural studies of Staufen1 in complex with Upf1, a crucial assembly in gene regulation, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1510371

Data is released under the CC-BY-4.0 license.