ISIS Neutron and Muon Source Data Journal

Tackling antimicrobial resistance: structural study of the Annexin A5/Lipopolysaccharide (LPS) complex

Abstract: Antimicrobial resistance is a global epidemic and has occurred due to a widespread antibiotic overconsumption. Gram negative bacteria, such as P. aeruginosa (leading cause of mortality in cystic fibrosis patients) are of particular concern due to the limited permeability of bacterial cell wall to antibiotic drugs. One promising strategy is to increase the permeability of the bacterial outer membrane prior to antibiotic delivery. Annexin A5 is a calcium-dependent phospholipid binding protein that targets phosphatidylserine (PS) and under certain conditions can destabilize membranes. Recently it has been shown to also bind LPS-containing membranes and here we propose to study the interaction of Annexin A5 with PS and LPS using neutron reflectivity in order to better understand the structural requirements to turn Annexin A5 into a potentially effective antimicrobial therapy agent.

Principal Investigator: Dr Filip Ciesielski
Experimenter: Dr Arwel Hughes
Experimenter: Dr Benjamin Davis
Experimenter: Dr Luke Clifton
Local Contact: Dr Maxmilian Skoda

DOI: 10.5286/ISIS.E.RB1510641

ISIS Experiment Number: RB1510641

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Filip Ciesielski et al; (2015): Tackling antimicrobial resistance: structural study of the Annexin A5/Lipopolysaccharide (LPS) complex, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1510641

Data is released under the CC-BY-4.0 license.