ISIS Neutron and Muon Source Data Journal

The structure of the Bam complex, the Gram-negative outer membrane protein folding machinery

Abstract: The proteins of the outer membrane of Gram-negative bacteria are critical for bacterial survival, from providing basic physiological functions, to impairing virulence and multidrug resistance. The Bam complex has been found to be essential for folding these proteins into the outer membrane. The process by which this is achieved is unknown. Key to this understanding is the knowledge of the structure of the complex and its individual components. We aim to continue our investigation of the Bam complex by studying the BamAB, BamCDE and complete BamABCDE complexes and in particular identify how the individual components are organised with respect to each other. To do this will use Small Angle Neutron Scattering and specific deuteration, in concert with a previously collected SANS dataset of the BamCDE sub complex, to probe the structure of the full Bam complex for first time.

Principal Investigator: Dr Tim Knowles
Experimenter: Dr Luke Clifton
Experimenter: Professor Michael Overduin
Experimenter: Dr Mark Jeeves
Local Contact: Dr Richard Heenan

DOI: 10.5286/ISIS.E.RB1510393

ISIS Experiment Number: RB1510393

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Tim Knowles et al; (2015): The structure of the Bam complex, the Gram-negative outer membrane protein folding machinery, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1510393

Data is released under the CC-BY-4.0 license.