ISIS Neutron and Muon Source Data Journal

Morphology and Phase Diagram of Thermoresponsive Polymer-Protein Conjugates

Abstract: Using an enzyme mediated conjugation method we have prepared well-designed protein-polymer conjugates using a model protein, green fluorescent protein (GFP) with thermo-responsive polymers based on HPMA (N-2- hydroxypropyl methacrylamide)-Acetyl-HPMA (O-acetyl-N-2-hydroxypropyl methacrylamide) and a commercially available PNIPAM-NH2.In this proposal we wish to study in greater detail the self-assembly of these temperature responsive polymer-protein conjugates. Such investigations could be particularly useful to understand the structural properties of these materials as the molecular weight changes and how they respond to the polymers transition to a collapsed state at higher temperatures. The phase diagram of eGFP-copolymer conjugates will be mapped out with respect to copolymer composition and concentration effects as well as temperature and water content.

Principal Investigator: Dr Amani El Fagui
Experimenter: Mr Matthew Learoyd
Experimenter: Professor Karen Edler
Experimenter: Dr Gavin Hazell
Experimenter: Dr Gabriel Cavalli
Experimenter: Dr Susanna Piluso
Experimenter: Ms Cecilia Tognoloni
Local Contact: Dr Richard Heenan

DOI: 10.5286/ISIS.E.RB1410433

ISIS Experiment Number: RB1410433

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Amani El Fagui et al; (2015): Morphology and Phase Diagram of Thermoresponsive Polymer-Protein Conjugates , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1410433

Data is released under the CC-BY-4.0 license.