ISIS Neutron and Muon Source Data Journal

Structural characterisation of a transmembrane biological electron conduit using SANS

Abstract: Many bacteria are able to respire using solid minerals and metals as electron acceptors in the absence of oxygen or other diffusible substrates. In Shewanella oneidensis this respiration requires a porin-cytochrome complex that assembles in the outer membrane. This complex is composed of a trans-membrane porin MtrB, a periplasmic deca-haem cytochrome MtrA and a surface exposed outer membrane cytochrome MtrC. We intend to obtain information on the shape and size of the MtrCAB complex, which will inform on the orientation of exposed MtrC as well as whether MtrA either fully or partially enters MtrB, thus providing evidence for MtrB functioning as a sheath for MtrA. We intend to perform small angle neutron scattering experiments using the SANS2D instrument to generate scattering profiles and ultimately a molecular surface model for this membrane protein complex.

Principal Investigator: Dr Tom Clarke
Experimenter: Dr Stephen Carr
Experimenter: Dr David Scott
Experimenter: Dr Marcus Edwards
Experimenter: Mr Matthew Lawes
Experimenter: Dr Gaye White
Experimenter: Dr DENIS PTCHELKINE
Experimenter: Miss Nishtha Gulati
Local Contact: Dr Sarah Rogers
Local Contact: Dr Richard Heenan

DOI: 10.5286/ISIS.E.RB1410125

ISIS Experiment Number: RB1410125

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Tom Clarke et al; (2015): Structural characterisation of a transmembrane biological electron conduit using SANS, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1410125

Data is released under the CC-BY-4.0 license.