ISIS Neutron and Muon Source Data Journal

Recreating the specific colicin N–Ra-LPS interaction in a model bacterial outer membrane

Abstract: The Gram negative bacterial outer membrane protects the bacterium from external toxins such as antibiotics. This is partially responsible for Gram negative bacteria becoming the greatest emerging source of antibiotic resistance. The outer membrane is itself the target of some antibacterial agents such as polymixins but even these are subject to resistance mechanisms. We recently discovered that another antibacterial protein, colicin N, binds to the outer membrane lipid in a novel way that centres on the core oligosaccharide rather than the anionic phosphate groups targeted by the polymyxin family. In a recent paper we showed the importance of electrostatics in this binding and revealed that specific binding needed faults in the outer membrane model which expose the binding site. In this project we hope to define how exposure of the sugars allows for specific membrane binding.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Nicolò Paracini
Experimenter: Dr Helen Waller
Experimenter: Dr Luke Clifton

DOI: 10.5286/ISIS.E.RB1620340

ISIS Experiment Number: RB1620340

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2016): Recreating the specific colicin N–Ra-LPS interaction in a model bacterial outer membrane, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1620340

Data is released under the CC-BY-4.0 license.