ISIS Neutron and Muon Source Data Journal

The Mechanism of Bam Mediated Outer Membrane Protein Folding

Abstract: The proteins of the outer membrane of Gram-negative bacteria are critical for bacterial survival,from providing basic physiological functions, to impairing virulence and multidrug resistance. The Bam complex has been found to be essential for folding these proteins into the outer membrane.The process by which this is achieved is unknown. The structure of the complex and its individual components are now known. We aim to continue our investigation of the Bam complex by studying BamABCDE in complex with stalled folding intermediates. To do this we will use specific deuteration and neutron reflectometry in concert with previous PNR datasets of the BamABCDE complex bound to a gold surface to probe the structure of the Bam complex as it performs its function.

Principal Investigator: Dr Tim Knowles
Experimenter: Miss Nadisha Gamage
Experimenter: Miss Caitlin Hatton
Experimenter: Dr Luke Clifton
Experimenter: Mr Peter Wotherspoon
Experimenter: Dr Stephen Hall
Experimenter: Dr Mark Jeeves

DOI: 10.5286/ISIS.E.RB1620475

ISIS Experiment Number: RB1620475

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Tim Knowles et al; (2016): The Mechanism of Bam Mediated Outer Membrane Protein Folding, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1620475

Data is released under the CC-BY-4.0 license.