ISIS Neutron and Muon Source Data Journal

Structural determination of SMA-lipid particles in solution

Abstract: The development of styrene-maleic acid lipid particles (SMALP) is opening the door to better understanding the nature of membrane proteins, these particles have been well characterised with non-atomistic techniques. However, the protein-free system is still not completely understood atomistically, with information such as the polymer conformation and the lipid arrangement still unknown. The ability of NIMROD to give atomic resolution on this organised system would allow the atomistic structure to be understood. Due to the large size of the system, we plan to use a pre-existing all-atom model as a starting point for the ESPR analysis, to assist in more rapidly determine the experimental solution structure. By comparing SMALPs formed using two different polymers we aim to understand polymer effects on encapsulated lipids which may impact properties of proteins supported in the nanodiscs.

Principal Investigator: Professor Karen Edler
Experimenter: Dr Ann Terry
Experimenter: Ms Cecilia Tognoloni
Experimenter: Dr Andrew McCluskey
Experimenter: Dr Joseph Curtis
Experimenter: Dr Daniel Bowron

DOI: 10.5286/ISIS.E.RB1620135

ISIS Experiment Number: RB1620135

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2016): Structural determination of SMA-lipid particles in solution, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1620135

Data is released under the CC-BY-4.0 license.