ISIS Neutron and Muon Source Data Journal

Polymer-phospholipid nanodiscs: Modification of polymer hydrophilicity.

Abstract: Polymer stabilized lipid nanodiscs offer enormous potential as tools for enabling membrane protein structural studies & biophysics. Previously we studied nanodisc formation using a poly(styrene-alt-maleic acid) copolymer, and also with a commercially available poly(styrene-alt-maleimide) (PSMI). We now wish to study the polymer chemistry in more detail to investigate the effect of structural changes to help determine how the disc structure is stabilized. We have used RAFT polymerisation to prepare well defined poly(styrene-alt-maleic acid) polymers with a diblock or triblock structure and also where the styrene has been partially sulfonated or instead incorporating acrylic rather than maleic acid. These variations in the hydrophilicity of the polymer alter the aggregate size in preliminary DLS studies but contrast variation SANS is needed to determine the structures formed with lipids.

Principal Investigator: Professor Karen Edler
Experimenter: Dr Stephen Hall
Experimenter: Miss Andi Di
Experimenter: Miss Naomi Elstone
Experimenter: Dr Thomas Arnold
Experimenter: Dr Andrew McCluskey
Experimenter: Dr Oliver Hammond
Local Contact: Dr James Doutch
Experimenter: Ms Cecilia Tognoloni
Experimenter: Dr Ann Terry
Experimenter: Dr Naomi Elstone

DOI: 10.5286/ISIS.E.RB1610317

ISIS Experiment Number: RB1610317

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Karen Edler et al; (2016): Polymer-phospholipid nanodiscs: Modification of polymer hydrophilicity., STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610317

Data is released under the CC-BY-4.0 license.