ISIS Neutron and Muon Source Data Journal

Adsorption of Fab and Fc from monoclonal antibody at the oil/water interface

Abstract: Following our previous study of adsorption of globular lysozyme and BSA, we continue to explore how different globular proteins adsorb at the oil/water interface by neutron reflection. Inter is preferred because we have built technical capability on this instrument. Fab and Fc are the constituents of a full mAb and we propose to study their adsorption before the whole mAb. Our specific aims are to (a) determine the actual thickness and volume fraction of Fab and Fc and (b) the effect of pH on the interfacial distribution. The results will enable us to compare their extent of distributions into the oil region with other globular proteins at the interface. The results will enable us to answer if all the globular proteins unfold and produce characteristic segment distributions into the oil region. This work will form the basis for us to study the adsorption of different antibodies.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Zongyi Li
Experimenter: Dr Mario Campana
Experimenter: Mr Zhiming Lu
Experimenter: Dr John Webster
Experimenter: Mr Ruiheng Li
Experimenter: Mr Sean Ruane

DOI: 10.5286/ISIS.E.RB1620046

ISIS Experiment Number: RB1620046

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2016): Adsorption of Fab and Fc from monoclonal antibody at the oil/water interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1620046

Data is released under the CC-BY-4.0 license.