ISIS Neutron and Muon Source Data Journal

SANS characterization of hybrid vesicles lipid/polybutadiene-polyethyleneoxide (DPPC/OB) for the insertion of membrane proteins

Abstract: Amphiphilic polymers are biocompatible, tunable and versatile macromolecules used in biotechnology and drug delivery. Similarly to the phospholipids that constitute the membrane of cells, they form bilayers in the form of vesicles (polymersomes or PS). PS can be used, for instance, to deliver drugs and release them according to certain stimuli (temperature, pH...). The properties of PS can be tuned by modifying the chemical structure of the polymer itself or by mixing them with lipid such as DPPC. We use mixtures of DPPC and PEGylated polymer in the form of hybrid vesicles to achieve the reconstitution of membrane proteins (MPs). Such MPs will be tested for directionality and functionality. Together with other techniques, SANS will help to characterize better the lipid/polymer bilayer. Such information will allow to tune the components to obtain the appropriate matrix to host MPs.

Principal Investigator: Dr Gianluca Bello
Experimenter: Miss Francesca Cavallini
Local Contact: Dr Sarah Rogers

DOI: 10.5286/ISIS.E.RB1620142

ISIS Experiment Number: RB1620142

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Dr Gianluca Bello et al; (2016): SANS characterization of hybrid vesicles lipid/polybutadiene-polyethyleneoxide (DPPC/OB) for the insertion of membrane proteins, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1620142

Data is released under the CC-BY-4.0 license.