ISIS Neutron and Muon Source Data Journal

Using neutron crystallography for investigating protonation states of a catalytic arginine residue from chlorite dismutase

Abstract: Chlorite dismutases (Clds) are metalloproteins containing heme b (i.e. Fe(III) in a porphyrin system) as redox cofactor. Clds are able to degrade toxic chlorite to chloride and dioxygen. This reaction is interesting from a biotechnological point of view as chlorite is a serious environmental pollutant and its bioremediation is an issue. However, mechanism of catalysis is not fully understood yet. Chlorite degradation activity is efficient at acidic pH, exhibiting its optimum at pH 5.0, but decreases significantly with rising pH. It is hypothesized that this depends, at least to a certain extent, on the protonation state of a conserved arginine residue. Investigating the protonation state of this residue under certain pH conditions by using neutron crystallography will highly contribute to understand Clds' reaction mechanism in general and the role of the conserved arginine in particular.

Principal Investigator: Professor Christian Obinger
Local Contact: Dr Pascal Manuel
Experimenter: Miss Irene Schaffner
Experimenter: Professor Kristina Djinovic-Carugo
Experimenter: Mr Georg Mlynek

DOI: 10.5286/ISIS.E.RB1520237

ISIS Experiment Number: RB1520237

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Christian Obinger et al; (2016): Using neutron crystallography for investigating protonation states of a catalytic arginine residue from chlorite dismutase, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1520237

Data is released under the CC-BY-4.0 license.