ISIS Neutron and Muon Source Data Journal

Assembling floating, very sparsely tethered, bilayers.

Abstract: The cell membrane is a very important structure in biology and many laboratory based model systems have been developed to study it using biophysical techniques. Our particular target is the bacterial outer membrane which is a significant barrier to antibiotics. Recently we have succeeded in making a realistic model but, to improve it further, we need to enlarge the water filled compartment below the model outer membrane which imitates the bacterial periplasm. Methods exist to anchor membranes above water layers but so far the density of anchoring molecule has been too high (>40%) to properly form a realistic periplasm. Here we wish to test a new very sparse anchoring method which works with a surface containing only 1% anchoring molecules. If this works we hope to extend the length of the spacers to control the size of our model periplasm.

Principal Investigator: Professor Jeremy Lakey [Deactivated] [Deactivated]
Experimenter: Dr Martynas Gavutis
Experimenter: Dr Luke Clifton
Experimenter: Dr Nicolò Paracini

DOI: 10.5286/ISIS.E.RB1610231

ISIS Experiment Number: RB1610231

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jeremy Lakey [Deactivated] [Deactivated] et al; (2016): Assembling floating, very sparsely tethered, bilayers. , STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610231

Data is released under the CC-BY-4.0 license.