ISIS Neutron and Muon Source Data Journal

Co-adsorption of monoclonal antibodies and non-ionic surfactants at the air/liquid interface

Abstract: Nonionic surfactants usually do not show affinity to proteins and they work via competitive surface and interfacial processes in a binary mixture with protein. However, we show in this work that Tween 80, a much bigger nonionic molecule than C12E5, with an oleyl chain, could initiate interaction possibly via the hydrophobic effect, evident from the surface tension plots. We propose to examine their surface co-adsorption processes by neutron reflection to gain direct structural evidence of the interaction, as this is a rather new observation from such systems. We request 4 days of Inter beam time (weak signal and low Q range needed) to exploit the contrast variations by using deuterated Tween 80 in combination with 3 different water contrasts (weak signals under some contrast combinations). The proposed measurements will enable us to analyse the structures via the kinematic approach.

Principal Investigator: Professor Jian Lu
Experimenter: Dr Chris van der Walle
Local Contact: Dr Mario Campana
Experimenter: Dr Peixun Li
Experimenter: Mr Robert Holman
Experimenter: Dr Lisa Pan
Experimenter: Dr John Webster
Experimenter: Dr Zongyi Li
Experimenter: Miss Daniela Ciumac
Experimenter: Mr Ruiheng Li

DOI: 10.5286/ISIS.E.RB1610215

ISIS Experiment Number: RB1610215

Publisher: STFC ISIS Neutron and Muon Source

Data format: RAW/Nexus
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Data Citation

The recommended format for citing this dataset in a research publication is as:
[author], [date], [title], [publisher], [doi]

For Example:
Professor Jian Lu et al; (2016): Co-adsorption of monoclonal antibodies and non-ionic surfactants at the air/liquid interface, STFC ISIS Neutron and Muon Source, https://doi.org/10.5286/ISIS.E.RB1610215

Data is released under the CC-BY-4.0 license.